Summary of a review by Lieshout et al 2019
A systematic review by Lieshout et al 2019 was recently published in the journal Critical Reviews in Food Science and Nutrition. The review focused on the effect of dairy protein modifications—caused by industrial heating—on its digestion and the overall physiological relevance of these modifications. The authors found that dairy processing significantly affects protein quality and can be used as a tool to steer gastrointestinal protein digestion.
Summary | The main protein modifications that occur during heat processing of milk are denaturation and aggregation of the protein and chemical modifications of its amino acids. Based on the findings of the systematic review by Lieshout et al 2019, many studies show that heat processing-induced protein modifications negatively impact protein digestion and overall protein quality. There is a need for more human studies to identify overall physiological relevance of digestive differences as a result of processing-induced protein modifications. In particular, the metabolic fate and longer-term impact of modified amino acids is, to a large extent, still unclear.
Dairy, especially milk, is a natural source of high quality protein. The high nutritional quality is attributed to the high level of essential amino acids and it’s high bioavailability. Industrial dairy processing can change the structure of milk proteins in several ways, depending on the conditions under which it was processed.
Characteristics of the review
The review by Lieshout et al 2019 is a systematic review on the effect of protein modifications on protein digestion in dairy. Via Scopus, 5,363 studies were retrieved and a total of 103 studies were included in the review. The studies were included when:
- Compared differently heat processed dairy proteins.
- Only major bovine dairy proteins were included.
- Digestion was involved that is (partly) representative for gastrointestinal digestion in humans, either in vivo or in vitro.
- Outcome measures related to protein digestion and absorption that were included were nitrogen balance calculations, protein hydrolysis, SDS-PAGE, LC/MS-MS data, or possible physiological consequences.
Review findings and discussions
During heat processing of milk and dairy products, glycation occurs. Glycation, also known as Maillard reaction, refers to a non-enzymatic reaction between sugars and proteins. According to the review, both animal and in vitro studies show that glycation decreases protein digestibility and amino acid availability, especially lysine. Possible explanations for the decrease in protein digestion are that glycation causes chemical modification of lysine residues. This then prevents cleavage by digestive enzymes (proteases).
Protein denaturation occurs during heat processing of milk and dairy products in the liquid phase. Unlike glycation, denaturation does not appear to affect overall protein digestibility and bioavailability. Nonetheless, protein denaturation can affect digestive kinetics mainly through an altered stomach behaviour. This is a possible explanation for the physiological consequences of heat-induced milk protein denaturation.
Other heat-induced chemical modifications can occur during heating of milk. Examples are oxidation, cyclization and racemization of amino acids. These modifications may also affect milk protein quality and digestibility, but are less studied. More research is needed to better understand their physiological effects.
Physiological effects for a change in digestibility or digestion kinetics mainly point towards amino acid bioavailability and immunological consequences. Processing-induced milk protein modification may alter their allergic potential through different processes with relevance for both sensitization and subsequent allergic response. Currently, the fate of modified amino acids after digestion is unclear and more data is needed.
The review findings are in line with the European Food and Safety Authority (EFSA)’s recommendation that milk processing in infant formulae should be kept as low as technologically possible owing to their potential effects on protein digestibility, protein metabolism, as well as immunological responses (EFSA Journal 2014;12(7):3760).
- Glenn A. A. van Lieshout, Tim T. Lambers, Marjolijn C. E. Bragt and Kasper A. Hettinga. How processing may affect milk protein digestion and overall physiological outcomes: A systematic review. Critical Reviews in Food Science and Nutrition DOI: 10.1080/10408398.2019.1646703
- EFSA Journal 2014;12(7):3760